BOD BOTTLES 500ML

Description

BOD Bottles (Biological Oxygen Demand) are specialized laboratory glassware designed specifically for the incubation of water samples. They are primarily used in environmental testing and wastewater analysis to determine the amount of dissolved oxygen consumed by microorganisms as they break down organic matter.

Key Product Features

Specialized Glass Stopper: Features a precision-ground, tapered glass robotic stopper. The “penny-head” or “robotic” design allows for a secure fit that creates an airtight seal.
Flared Mouth Design: The top of the bottle neck is flared to form a “water seal.” When a small amount of water is poured into the flare, it prevents atmospheric air from entering the bottle during the 5-day incubation period.
Borosilicate 3.3 Glass: Manufactured from high-quality, chemically resistant glass that can withstand repeated sterilization and incubation cycles without leaching or degradation.
Individual Numbering: Most 500ml BOD bottles come with a permanent, large-font sequence number fired into the glass. This ensures easy identification and tracking of multiple samples within an incubator.
White Marking Spot: Includes a permanent matte finish area for labeling with pencil or marker.

Technical Specifications

Feature	Details
Capacity	500ml
Material	Borosilicate 3.3 Glass
Stopper Type	Tapered Glass (Robotic/Penny-head)
Body Style	Cylindrical with shoulder and flared neck
Accuracy	Designed for “Full-Bottle” incubation (no headspace)
Cleaning	Autoclave-safe; resistant to harsh detergents

Application in BOD Testing

Sample Collection: The 500ml sample is collected without trapping air bubbles.
Sealing: The glass stopper is inserted. The tapered design forces any excess liquid out, ensuring zero headspace (no air bubbles) inside the bottle.
Incubation: A water seal is added to the flared neck, and a plastic cap is often placed over the top to prevent evaporation of the seal.
Measurement: The sample is incubated (typically at 20°C for 5 days). The difference in dissolved oxygen levels before and after incubation determines the BOD value.

Why 500ml vs. 300ml?

While the 300ml BOD bottle is the standard for many regulatory tests, the 500ml version is preferred when dealing with:

Low BOD Samples: Larger volumes provide higher sensitivity for samples with very low organic content (e.g., treated drinking water or clean river water).
Sub-Sampling: When multiple chemical tests need to be run from the same incubated volume.
Wastewater Analysis: Providing a more representative sample size for heterogeneous mixtures.

Care and Handling

Stopper Integrity: Always check that the ground-glass joint of the stopper and the neck are clean and free of chips to ensure a perfect airtight seal.
Cleaning: Thoroughly clean with non-phosphate detergents and rinse with distilled water to remove any traces of organic matter that could skew test results.

Additional information

Cap	500ml

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